Reporter mRNA
Our reporter mRNAs are optimised and validated for strong, durable expression, and can be used for reliable and quantitative assessment of mRNA delivery, translation, and stability.
mCherry mRNA expression in HEK293 cells
eGFP mRNA expression in HEK293
tdTomato mRNA expression in HEK293 cells
mRNA catalogue
Reporter mRNAs
eGFP
Gold-standard monomeric green fluorescent protein (Ex 488 nm / Em 509 nm, 26.9 kDa, ~720 nt) from Aequorea victoria, ideal for transfection optimisation, protein localisation, and flow cytometry.
Luciferase (FLuc)
Widely-used bioluminescent reporter enzyme (peak ~560 nm, 62 kDa, ~1,650 nt) derived from Photinus pyralis, requiring ATP and d-luciferin substrate, suited to mRNA delivery quantification and in vivo imaging.
mCherry
Fast-maturing monomeric red fluorescent protein (Ex 587 nm / Em 610 nm, 26.7 kDa, ~711 nt) from Discosoma mRFP1, with durable photostability suited to multi-colour imaging and long-duration, live-cell experiments.
mScarlet
Bright monomeric red fluorescent protein (Ex 569 nm / Em 594 nm, ~26 kDa, ~699 nt) developed from a synthetic consensus template, suited to quantitative imaging and FRET applications.
tdTomato
Bright tandem-dimer orange-red fluorescent protein (Ex 554 nm / Em 581 nm, ~54 kDa, ~1,380 nt) from Discosoma DsRed, suited to endosomal and lysosomal tracking due to low acid sensitivity.
Vaccine mRNAs
Ovalbumin
A glycoprotein derived from Gallus gallus egg white (42.7 kDa, 385 amino acids, ~1,158 nt) and gold-standard model antigen for vaccine immunology, eliciting both humoral and cellular immune responses and enabling CD4+ and CD8+ T cell studies.
HA/H1N1
A surface glycoprotein from influenza A H1N1 (~59 kDa, 566 amino acids, ~1,700 nt) mediating host cell receptor binding and membrane fusion; the primary target for neutralising antibodies and standard model antigen for mRNA influenza vaccine development and assays.
Spike
Full-length prefusion-stabilised spike glycoprotein from SARS-CoV-2 (~140 kDa, 1,273 amino acids, ~3,825 nt), and a primary antigen for vaccine research and neutralising antibody studies.
SIINFEKL
Immunodominant MHC class I-restricted peptide epitope (~1 kDa, 24 nt) derived from chicken ovalbumin (OVA residues 257–264) serves as a standard model epitope for CD8+ T cell activation, tetramer studies, ELISPOT, and cytotoxicity assays.
Gene-editing mRNAs
Cas9
Cas9 (~160 kDa, 1,368 amino acids, ~4,107 nt) directs double-strand DNA cleavage via sgRNA. mRNA delivery suited to transient, non-integrating CRISPR-Cas9 delivery for gene knockouts, insertions, deletions in cells and animal models.
Cre recombinase
Tyrosine recombin (~39 kDa, 343 amino acids, ~1,032 nt) that catalyses DNA excision, inversion, or translocation between paired loxP recognition sites; suited to transient mRNA delivery for conditional gene knockouts, lineage tracing, and genomic rearrangements in Cre/loxP transgenic mouse models.
Specifications
Shipping
Dispatched in 1–2 days
Scale
100 µg to 10+ mg
Design
Design
mRNArchitect (max CAI, min MFE)
UTRs
human beta-globin
Poly(A) tail
126nt
Production
Nucleotides
m1Ψ
5′ cap
Co-transcriptional Cap-1
DNA template
PCR (cell-free)
mRNA synthesis
IVT using T7 polymerase
mRNA purification
oligo(dT)
LNPs
Standard
Quality control
mRNA yield
UV
mRNA integrity
Capillary Gel Electrophoresis
Additional purity
dsRNA, endotoxin
LNP quality
DLS, encapsulation
Cy5 mRNA
Our Cy5-labelled mRNA (red) can be directly visualised, allowing tracking of the cellular uptake, intracellular distribution, through to mRNA translation. Co-localisation with eGFP expression (green) shows translation within the cell.
Cy5-labelled eGFP mRNA in HEK293 cells
mRNA translation in HEK293 cells
Merged image showing DAPI-stained HEK293 nuclei
Enquiries
Our team is available to help with your mRNA projects.