SERVICES.
The BASE facility makes mRNA and dsRNA.
The BASE facility can provide a quote according to any project requirements.
We provide end-to-end services to support academic, translational and commercial research.
Project design. BASE tailors project design and deliverables according to customer requirements. We have experience in manufacturing different mRNA types, and can help with the design, manufacture and formulation of your mRNA drug.
Construct design and cloning. We can help design optimised mRNA sequences that are cloned into production vectors. We have established a modular approach for rapid and complex plasmid assembly that supports efficient candidate screening.
Upstream processes. We have established processes for amplification, purification and linearization of the plasmid DNA. This template is then in vitro transcribed into the mRNA (or dsRNA) product. We also provide 5’ capping and modified nucleotides. We manufacture small-scale batches for pilot research studies, or large-scale for translational and commercial outcomes.
Downstream processes. BASE has established downstream processes using chromatographic and filter-based processes to purify final mRNA products. Our mRNA processes use industry standards to ensure seamless translation and scale-up for future production or clinical phases.
Analytics. BASE uses next-generation sequencing to measure the quality of RNA and DNA at each production step, and will provide a detailed report confirming the analysis of full-length sequence, integrity and purity.
Formulation. BASE offers a formulation of mRNA within lipid nanoparticles. We can also provide collaborative options for a range of novel delivery technologies developed at the AIBN.