Custom mRNAs

Designed to specification.

Building mRNAs

We manufacture custom mRNAs according to your design specifications and ready to use in your experiments.

Our end-to-end services include;

mRNA design
DNA template and mRNA synthesis (from 100ug to 1g)
LNP formulation

Our expert team can provide help at each step in the process.

mRNA products

Off-the-shelf mRNA

Small-scale custom mRNA for in vitro studies.

Best choice for cell culture experiments.
Custom mRNA made to spec.
mRNA design (CDS & UTR)
DNA template synthesis.
in vitro transcription and purification.
Analytical report.
Ready for transfection into cell culture.

Large-scale custom mRNA for in vivo studies

mRNA Services

The BASE facility has experience making mRNA for a wide variety of applications.

Our mRNA is high-quality – supported by rigorous quality control and validation in many experiments and cell-types.

Project design

We can provide useful advice and protocols for using mRNA in cell and mouse studies.

mRNA Sequence Design

We can design mRNAs from any open reading frame, as well as providing validated UTRs and polyA tails for robust expression.

DNA Template

We synthesise and validate DNA templates according to your approved design.

mRNA Synthesis

We can synthesise mRNA a different scales (0.1-1g), with inclusion of 5’caps and modified nucleotides.

LNP Formulation

We can formulate your mRNA within lipid nanoparticles (LNPs) for effective in vivo delivery.

Quality Control

All of our mRNA is rigorously tested to ensure quality control, with results provided in a detailed report.

Quality Feature	Method
Sequence Identity (DNA template)	NGS, Sanger
mRNA Purity	UV Spectrophotmetry
mRNA Length / Integrity	Capillary Electrophoresis
mRNA Concentration	UV Spectrophotmetry
mRNA Legnth / Integrity	HPLC
Appearance	Visual Inspection

mRNA quality control

Our mRNA undergoes rigorous quality control testing.

Each mRNA batch is accompanied by an analytical report measuring purity and integrity.

Consistent quality controls ensure the reliability of mRNA and reproducibility of your experiments.

LNP quality control

We offer scalable LNP formulation of mRNAs for in vivo delivery in animal studies.

Each mRNA/LNP batch undergoes rigorous analysis using our suite of quality control tests.

Quality Feature	Method
mRNA Encapsulation	Ribogreen Assay
Particle Size / Uniformity	Dynamic Light Scattering
Zeta potential	Dynamic Light Scattering
Endotoxins	LAL Assay
Appearance	Visual Inspection

Rapid mRNA production

Watch our webinar with Dr Seth Cheetham, in partnership with Integrated DNA Technologies (IDT), shows how to quickly DNA templates can be made for synthesis of mRNA for pre-clinical research.

Enquiries

Our support team is available to help with your mRNA projects.